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six1xenopus otic region 

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Experiment details for six1

Hairy2-Id3 interactions play an essential role in Xenopus neural crest progenitor specification.

Hairy2-Id3 interactions play an essential role in Xenopus neural crest progenitor specification.

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Gene Clone Species Stages Anatomy
six1.L laevis NF stage 22 otic region

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  Figure S4. Hairy2 overexpression does not induce major cell fate changes in the early embryo. (A) In neurula embryos, Hairy2-GR mRNA (500 pg) injection represses the expression of the MyT1, N-tubulin and NeuroD neuronal markers (a, 50% n = 22; b, 55% n = 18; c, 52% n = 27). No significant change is observed on the expression of neural plate (Sox3), mesodermal (MyoD), placodal (Six1, Six3, Pax8) and antero-posterior patterning markers (Otx2, HoxB9, XAG1). All unaffected: d, n = 15; e, n = 34; f, n = 14; g, n = 17; h, n = 24; i, n = 25; j, n = 16; k, n = 25; l, n = 20. Hairy2-GR mRNA (500pg) represses the epidermal marker Epi. K. (m, 64% n = 26 and expands transiently Sox2 at neurula stages (n, 66% increase n = 18) but have no effect on Sox2 at tailbud stages (o, 100% unaffected n = 19). (B) Hairy2-GR overexpression (500 pg) decreases N-tubulin (a, 75% n = 12; b, 53% n = 19; c, 55% n = 20; d, 50% n = 12) and does not affect Sox3 and Six1 expression in stage 13 to 26-27 embryos. (e-l, all unaffected; n = 20). Embryos are shown in dorso- anterior views with the injected side to the right (a-n) or in lateral view with inset showing the control side (o). β-galactosidase was used as a lineage tracer. (C) Q-PCR analysis of stage 14 NC induced animal caps derived from embryos injected with Hairy2 MO5mis (5 ng/blastomere), Hairy2 MOs (5 ng/blastomere) or Hairy2-GR mRNA (250 pg/blastomere). Note that both Hairy2 gain or loss of function reduces Slug (a) without significantly affecting Six1, Sox3 or N-tubulin expression. Ep. keratin expression which is strongly reduced in NC induced explants, appears also unaffected by Hairy2(c).
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