|
|
|
Fig. 3. Expression of Six3 subfamily members during Xenopus head development. (A,F) Frontal views of stage 20 embryos showing Six3.2 (A) and Six6.1 (F) expression in the optic field (op) and future ventral forebrain (arrow). (B–D,G–I) Lateral views of embryonic stages 30 (B,G), 35 (C,H) and 39 (D,I) stained for Six3.2 (B–D) and Six6.2 (G–I). The arrowhead indicates the pineal gland primordium and the arrow highlights the olfactory bulb. (E,J) Transverse head sections of stage 35 embryo at the level of the cement gland illustrating Six3.2 (E) and Six6.2 (J) expression. The developing lens (arrowhead), the ventral floor of the diencephalon (de) and the dorsal portion of Rathke's pouch (arrow) are indicated. (K,L) Transverse sections through the head of stage 39 embryos stained for Six3.2 (K) and Six6.2 (L). The insets illustrate Six gene expression in the eye. Arrowheads indicated head mesenchyme. Abbreviations: cg, cement gland; gcl, ganglion cell layer; inl, inner nuclear layer; lv, lens vesicle; op, optic field; os, optic stalk; p, pharyngeal endoderm; rpe, retinal pigment epithelium; tg, tegmentum. |
|
|
|
six6 (SIX homeobox 6) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 20, anterior view, dorsal up |
|
|
|
six6 (SIX homeobox 6) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 29 & 30, lateral view, anterior left, dorsal up.
Key:os= optic stalk; lv= lens. |
|
|
|
six6 (SIX homeobox 6 ) gene expression in X. laevis embryo, head region, NF stage 29 & 30, lateral view. dorsal up, anterior left. |
|
|
|
six6 (SIX homeobox 6) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35 & 36, lateral view, anterior left, dorsal up. |
|
|
|
sx6 (SIX homeobox6) gene expression in Xenopus laevis embryo, transverse section through trunk region, assayed via in situ hybridization, NF stage 35 & 36, dorsal up.
Key: de= diencephalon floor plate; arrow: Rathke's pouch |
|
|
|
six3 (SIX homeobox 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 39, lateral view, anterior left, dorsal up |
|
|
|
six6 (SIX homeobox 6) gene expression in Xenopus laevis embryo, transverse sections through the head, assayed via in situ hybridization, NF stage 39, dorsal up, and higher magnification of sectioned eye (right).
Key: rpe: retinal pigmented epithelium; inl= inner nuclear layer; gcl= ganglionic cell layer, p=pharyngeal endoderm; arrowhead: head mesenchyme.
|