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Figure 2.
Expression Patterns of XOptx2 during Eye and Embryonic Development
(A–C), (F), and (G) are anterior views with dorsal (d) to the top, while (D) and (E) are lateral and dorsal views of the same embryo with anterior (a) to the right. In (F), XOptx2 is detected in the pineal gland primordia (arrowhead) and the ventral forebrain (arrow) as well as the eyes. In (G), the brown area is the pigmented cement gland.
(H–M) Expression patterns on sections of XOptx2 during eye development. (H–K) XOptx2 is expressed in the ventral forebrain/diencephalon (Dien), optic vesicle (ov), optic cup (oc), neural retina (nr), and the optic stalk (os) but is undetectable in the ectoderm (ect), the lens placode (lp), and the lens vesicle (lv). Sections of stage 41 eyes (L and M) were costained to detect XOptx2 (blue) and rhodopsin (red) expression. XOptx2 is detected in the ganglion cell (gcl) and inner nuclear cell (inl) layers but is absent from the photoreceptor (pr) layer. In (L) and (M), both the immature section of the ventral retina (arrowhead) and the ciliary marginal zone (cmz) express XOptx2. The nonspecific signal in stage 41 section (M) is autofluorescence of the lens (l). Additional abbreviations: anr, anterior neural ridge; p, posterior; v, ventral; ef, eye field; mid, midline; ppe, primordial pigment epithelium; pe, pigment epithelium. Scale bars = 300 μm (A–C, F, H, and I), 600 μm (D and E), 500 μm (G), and 100 μm (J–M). |
