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Fig. 1.
Synteny conservation around six6 loci in Xenopus tropicalis, X. laevis, and Homo sapiens, and quantitative divergence in the expression of X. laevis six6 homeologs. (A) Diagram of orthologous or homeologous chromosomal regions around X. tropicalis six6, X. laevis six6.L and six6.S, and H. sapiens SIX6. Pentagonal arrows represent genes and their transcriptional directions, and those with the same color are orthologs or homeologs. Gene names are indicated only on the X. tropicalis chromosome, except for six6 genes. The gene named c14orf39 in H. sapiens was named LOC100486324 in the X. tropicalis genome assembly. *Gene models for X. laevis c14orf39/ LOC100486324 are absent from the genome assembly, but a BLAST search with X. tropicalis LOC100486324 identified partial coding sequences of X. laevis orthologs in the corresponding regions of chromosomes 8L and 8S. (B) RNA-seq analysis of six6.L and six6.S using whole embryos at various developmental stages. Graph label suffixes _T and _U indicate the duplicated data sets Taira [201 203] and Ueno [201,210], respectively. (C) In situ hybridization analysis of X. tropicalis six6, X. laevis six6.L, and six6.S expression in tailbud embryos. Arrowheads and arrows indicate expression in the retina and optic stalk, respectively. |
