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slc31a1xenopus midbrain [+] 

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Experiment details for slc31a1

Vertebrate Ctr1 coordinates morphogenesis and progenitor cell fate and regulates embryonic stem cell differentiation.

Vertebrate Ctr1 coordinates morphogenesis and progenitor cell fate and regulates embryonic stem cell differentiation.

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Gene Clone Species Stages Anatomy
slc31a1.L laevis NF stage 32 midbrain

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  Fig. 3. Xctr1 regulates Ras-ERK signaling. (A) Coprecipitation of Xctr1, Laloo, and the docking protein, SNT-1/FRS2α. (B) Xctr1 knockdown inhibits Laloo-mediated SNT-1 phosphorylation (0.37 ± 0.11-fold reduction by Xctr1MO; n = 3). Signal represents the phosphorylation of up to six tyrosine residues on SNT-1 (10–12). (C) Xctr1 knockdown inhibits ERK phosphorylation (0.62 ± 0.27-fold reduction by MO; n = 4). Effects of injection of Xctr1MO and Xctr1MM on the FGF-mediated dual phosphorylation (dp) of the ERK MAP kinase in animal cap explants. Explants were cultured in the presence of FGF for 2 h before Western blot analysis; inhibition of ERK phosphorylation by Xctr1MO was not apparent for the first hour of FGF stimulation (data not shown). (D) Inhibition of activin-mediated dorsal mesoderm induction in animal cap explants by 1 ng of dominant inhibitory Ras (dnRas) RNA (1). (E) Rescue of Xctr1 morpholino-mediated dorsal mesoderm inhibition by coinjection of 40-pg constitutively active Ras (v-Ras) RNA (1). (F) Injection of 1 ng dnRas RNA does not enhance elongation of animal caps by FGF. Ras inhibition also inhibits elongation in FGF-treated caps from embryos injected with Xctr1MO (data not shown). (G) RT-PCR analysis of Xctr1 expression in early stage Xenopus embryos. (H) Whole-mount in situ hybridization analysis of Xctr1 expression. Stages (Upper) 4, 8 (animal views), 18 (dorsal view), and (Lower) 32 (lateral view) are shown. Pronephric tubules are indicated by arrowhead; 250-ng morpholinos were injected at early cleavage stages as listed, and 10 ng/ml FGF and 0.5 ng/ml activin were added to animal cap explants at stage 8 as listed.