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Fig. 2. A. Northern blot analysis of poly-A RNA isolated from multiple tissues from the adult mouse (left panel) and mouse embryo (middle panel) and
Xenopus stage 11 embryo (right panel). Blots were hybridized with the respective species-specific MUprobe. Left panel (each lane contains 2 pg RNA)
shows testis (lane I), kidney (2), skeletal muscle (3). liver (4), lung (51, spleen (6). brain (7) and heart (8); middle panel. 17 (lane l), 15 (2). 11 (3) and 7
days (4) old mouse embryo; right panel, Xenopus stage 11 embryo. (B) In situ hybridization analysis of MLP expression in sections of the mouse embryo
(left panel) and whole-mount Xenopus embryos (right panel). The left panel shows sagittal sections of a 9.5 days p.c. embryo (upper part of this panel)
and two sections of a 12.5 days p.c. embryo (middle and lower part of this panel, which are transversal and sagittal sections, respectively). Note the
uniform expression pattern in the E9.5 and the strongly elevated MusMLP expression in gonads and in mesenchymal cells lining the lung epithelium of
the El25 embryo. For a descriptive summary, see text: ba, branchial arches: du, Mulletian or Wolffian duct; go, gonads: lu. lung; so, somites; sp, spleen;
vb, vertebrae. Right panel: (A) stage 10 (early gastrula) Xenopus embryo shown from the vegetal side, dorsal side up; (B) is a stage 30 (tadpole) embryo
in lateral view: ba, branchial arches: br. brain: sn, spinal nerves. |
