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smad1xenopus   

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Experiment details for smad1

Cibois M et al. (2015) Assay

BMP signalling controls the construction of vertebrate mucociliary epithelia.

Gene Clone Species Stages Anatomy
smad1.L laevis NF stage 9 presumptive ectoderm , nucleus
smad1.L laevis NF stage 10 ectoderm , ectodermal cell , nucleus
smad1.L laevis NF stage 12 ectoderm , ectodermal cell , non-neural ectoderm , nucleus
smad1.L laevis NF stage 12 ectoderm , epithelium , ectodermal cell , epithelial cell , non-neural ectoderm , [+]
smad1.L laevis NF stage 15 ectoderm , epidermis , ectodermal cell , non-neural ectoderm , nucleus
smad1.L laevis NF stage 15 ectoderm , epidermis , epithelium , ectodermal cell , epithelial cell , [+]
smad1.L laevis NF stage 15 ectoderm , epidermis , epithelium , ectodermal cell , epithelial cell , [+]
smad1.L laevis NF stage 15 ectoderm , epidermis , epithelium , ectodermal cell , epithelial cell , [+]
smad1.L laevis NF stage 17 ectoderm , epidermis , ciliated epidermal cell , epithelium , ectodermal cell , [+]
smad1.L laevis NF stage 17 ectoderm , epidermis , epithelium , ectodermal cell , ionocyte , [+]
smad1.L laevis NF stage 17 ectoderm , epidermis , epithelium , ectodermal cell , epithelial cell , [+]
smad1.L laevis NF stage 19 ectoderm , epidermis , ectodermal cell , non-neural ectoderm , nucleus
smad1.L laevis NF stage 19 ectoderm , epidermis , ciliated epidermal cell , epithelium , ectodermal cell , [+]
smad1.L laevis NF stage 19 ectoderm , epidermis , epithelium , ectodermal cell , epithelial cell , [+]
smad1.L laevis NF stage 25 ectoderm , epidermis , epidermis outer layer , ciliated epidermal cell , epithelium , [+]
smad1.L laevis NF stage 25 ectoderm , epidermis , epidermis outer layer , epithelium , ectodermal cell , [+]
smad1.L laevis NF stage 25 ectoderm , epidermis , epidermis inner layer , ectodermal cell , non-neural ectoderm , [+]
smad1.L laevis NF stage 32 ectoderm , epidermis , epidermis outer layer , ciliated epidermal cell , epithelium , [+]
smad1.L laevis NF stage 32 ectoderm , epidermis , epidermis outer layer , neuroectoderm , epithelium , [+]
smad1.L laevis NF stage 32 ectoderm , epidermis , epidermis inner layer , epithelium , ectodermal cell , [+]

Display additional annotations [+]
  Fig. 1. BMP pathway activity in the developing Xenopus epidermis. (A) Immunostaining of cryosectioned Xenopus embryos revealed that the pSmad1/5/8 signal (red) colocalises with nuclear DAPI staining (blue) in both inner and outer layer cells. (B) (Left) In embryos injected with linearised plasmid DNA for the MCC reporter α-tub::GFP (50 pg/embryo), the pSmad1/5/8 signal (red) colocalised with GFP immunostaining (green). (Middle) In embryos injected with linearised plasmid DNA for the ionocyte reporter pendrin::GFP (25 pg/ embryo), the pSmad1/5/8 signal (red) colocalised with GFP immunostaining (green). (Right) The pSmad1/5/8 signal (red) colocalised with P63 protein expression (white). For each marker and stage, a representative cell (yellow arrowhead) is displayed at higher magnification to show the double staining. The white dashed and dotted lines respectively mark the apical surface of the ectodermal outer layer and the boundary between the outer and the inner ectodermal layers.

Gene Clone Species Stages Anatomy
smad1.L laevis NF stage 9 to NF stage 10 ectoderm , nucleus

Display additional annotations [+]
  Figure S1: Validation of pSmad1/5/8 staining in response to BMP manipulation. (A): Injection of stage 9 Xenopus embryos with recombinant BMP4 led to increased Smad1/5/8 phosphorylation and nuclear localization. (B): The percentage of nuclei positive for the pSmad signal is significantly higher in BMP-treated than in control embryos (Student's t-test). (C): Knocking down BMP2, BMP4 and BMP7 with specific morpholino oligonucleotides inhibited Smad1/5/8 phosphorylation. Embryos were injected with either GFP mRNA alone (upper row) or GFP mRNA and the BMP2/4/7 MOs (lower row). The pSmad1/5/8 signal was lost in cells that received the BMP2/4/7 MOs, but not in those that received only the GFP mRNA.