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smad1xenopus spinal nerve 

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Experiment details for smad1

The C-terminal domain of Mad-like signal transducers is sufficient for biological activity in the Xenopus embryo and transcr...

The C-terminal domain of Mad-like signal transducers is sufficient for biological activity in the Xenopus embryo and transcriptional activation.

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Gene Clone Species Stages Anatomy
smad1.S laevis NF stage 29 and 30 spinal nerve

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  Fig. 2. A. Northern blot analysis of poly-A RNA isolated from multiple tissues from the adult mouse (left panel) and mouse embryo (middle panel) and Xenopus stage 11 embryo (right panel). Blots were hybridized with the respective species-specific MUprobe. Left panel (each lane contains 2 pg RNA) shows testis (lane I), kidney (2), skeletal muscle (3). liver (4), lung (51, spleen (6). brain (7) and heart (8); middle panel. 17 (lane l), 15 (2). 11 (3) and 7 days (4) old mouse embryo; right panel, Xenopus stage 11 embryo. (B) In situ hybridization analysis of MLP expression in sections of the mouse embryo (left panel) and whole-mount Xenopus embryos (right panel). The left panel shows sagittal sections of a 9.5 days p.c. embryo (upper part of this panel) and two sections of a 12.5 days p.c. embryo (middle and lower part of this panel, which are transversal and sagittal sections, respectively). Note the uniform expression pattern in the E9.5 and the strongly elevated MusMLP expression in gonads and in mesenchymal cells lining the lung epithelium of the El25 embryo. For a descriptive summary, see text: ba, branchial arches: du, Mulletian or Wolffian duct; go, gonads: lu. lung; so, somites; sp, spleen; vb, vertebrae. Right panel: (A) stage 10 (early gastrula) Xenopus embryo shown from the vegetal side, dorsal side up; (B) is a stage 30 (tadpole) embryo in lateral view: ba, branchial arches: br. brain: sn, spinal nerves.