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smad1xenopus animal hemisphere [+] 

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Experiment details for smad1

Xenopus mothers against decapentaplegic is an embryonic ventralizing agent that acts downstream of the BMP-2/4 receptor.

Xenopus mothers against decapentaplegic is an embryonic ventralizing agent that acts downstream of the BMP-2/4 receptor.

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Gene Clone Species Stages Anatomy
smad1.L laevis NF stage 10 animal hemisphere

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  Fig. 2. Developmental expression of Xenopus Mad. (A) A developmental northern blot of total embryonic RNA shows that XMad is maternal and expressed at all stages of early development. Three transcripts are detected with the sizes (in kb) indicated on the left. The most abundant mRNA is 3.4 kb, and minor transcripts of 4.3 kb and 3.0 kb are also detected. It is not known whether these transcripts derive from closely related genes or splicing variants. Lane numbers correspond to developmental stages (Nieuwkoop and Faber, 1967): (7, 9) blastula, (11) gastrula, (15, 18) neurula, (26) tailbud tadpole and (38) swimming tadpole. (B) In situ hybridization shows that XMad transcripts are uniformly distributed in the early gastrula (stage 10). Dense staining covers the entire prospective ectoderm and marginal zone, but vegetal cells (the lighter region) do not stain efficiently by this procedure (Harland, 1991). A northern blot on isolated dorsal, ventral, animal and vegetal regions confirmed the uniformity of XMad expression at early stages (not shown). (C) A mid-gastrula embryo (stage 12) split sagitally reveals that XMad is expressed in the ectoderm and neurectoderm (arrows mark the ectodermal-mesodermal boundary), and expression in the underlying mesoderm is greater in the posterior, adjacent to the yolk plug (YP). This embryo is lightly pigmented and the brown line anterior to the yolk corresponds to involuted bottle cells. The embryo is positioned with the anterior to the left and dorsal at the top. (D) At tailbud tadpole stage 26 XMad expression is high in the central nervous system and head. (E) A close-up of the head of the embryo in D, highlighting XMad expression in the brain (b), eye (e) and head neural crest derivatives (mc, mandibular crest; hc, hyoid crest; abc, anterior branchial crest; pbc, posterior branchial crest). Expression in the otic vesicle, between the hyoid crest and anterior branchial crest, is also visible. Scale bars, 0.1 mm.