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smad1xenopus epithelial cell 

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Experiment details for smad1

Cibois M et al. (2015) Assay

BMP signalling controls the construction of vertebrate mucociliary epithelia.

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Gene Clone Species Stages Anatomy
smad1.L laevis NF stage 12 epithelial cell
smad1.L laevis NF stage 15 epithelial cell , epidermal cell
smad1.L laevis NF stage 15 epithelial cell , ionocyte
smad1.L laevis NF stage 15 epithelial cell , epidermal cell
smad1.L laevis NF stage 17 ciliated epidermal cell , epithelial cell , epidermal cell
smad1.L laevis NF stage 17 ionocyte
smad1.L laevis NF stage 17 epithelial cell , epidermal cell
smad1.L laevis NF stage 19 ciliated epidermal cell , epithelial cell , epidermal cell
smad1.L laevis NF stage 19 epithelial cell , ionocyte , epidermal cell
smad1.L laevis NF stage 25 ciliated epidermal cell , epithelial cell , epidermal cell
smad1.L laevis NF stage 25 epithelial cell , ionocyte , epidermal cell
smad1.L laevis NF stage 25 epidermal cell
smad1.L laevis NF stage 32 ciliated epidermal cell , epithelial cell , epidermal cell
smad1.L laevis NF stage 32 epithelial cell , ionocyte , epidermal cell
smad1.L laevis NF stage 32 epithelial cell , epidermal cell

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  Fig. 1. BMP pathway activity in the developing Xenopus epidermis. (A) Immunostaining of cryosectioned Xenopus embryos revealed that the pSmad1/5/8 signal (red) colocalises with nuclear DAPI staining (blue) in both inner and outer layer cells. (B) (Left) In embryos injected with linearised plasmid DNA for the MCC reporter α-tub::GFP (50 pg/embryo), the pSmad1/5/8 signal (red) colocalised with GFP immunostaining (green). (Middle) In embryos injected with linearised plasmid DNA for the ionocyte reporter pendrin::GFP (25 pg/ embryo), the pSmad1/5/8 signal (red) colocalised with GFP immunostaining (green). (Right) The pSmad1/5/8 signal (red) colocalised with P63 protein expression (white). For each marker and stage, a representative cell (yellow arrowhead) is displayed at higher magnification to show the double staining. The white dashed and dotted lines respectively mark the apical surface of the ectodermal outer layer and the boundary between the outer and the inner ectodermal layers.