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Fig. 3. Expression of XSmad2 and XSmad3 mRNA detected by in situ hybridization. In situ hybridization using probes specific for XSmad2 (A,C,E,G) or XSmad3 (B,D,F,H–J) on embryos of different developmental stages (indicated at the left). The specific staining is blue. In (A,B), Stage 10.25 embryos were cut in half prior to in situ hybridization analysis, and arrow head indicates the position of the dorsal blastopore lip. In (D,F,H), arrows indicate either the heart staining (Ht) or the chordoneural hinge staining (CNH). Boxed areas in panel (F) are shown magnified in panels (I,J). |
