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Fig. 7. Xphb1 depletion does not shift the neural plate border. (A-C) The expression of zic3, pax3 and meis3 at stage 14 was not affected after phb1 MO (*) injection. (D,E) cytokeratin Xk81A1 (D, stage 16) and sox2 (E, stage 18) expression areas remained unchanged upon phb1 MO injection. (F) In double ISH, the snail1 (purple) expression area facing the sox2 (red) region showed a normal pattern after Xphb1 depletion (arrowhead marks β-gal staining). (G) Double ISH for sox2 (red) and snail2 (purple), which is lost on the phb1 MO-injection side. |
