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Figure 4. mZIP impairs maintenance of retinotectal synapses in vivo. A, Representative immunostaining of the tectal neuropil from a horizontal brain section with antibodies against SNAP-25 (red) and PSD-95 (green) 4 h after tectal infusion of vehicle (control) and mZIP. Yellow puncta represent the overlap of SNAP-25 and PSD-95 puncta. Scale bars, 1 μm. B, C, Quantitative analysis of the density of SNAP-25 puncta, PSD-95 puncta, and juxtaposed puncta (B), as well as colocalization probability (C), normalized by the average value of the corresponding controls (n = 5 tadpoles for each condition; *p < 0.05). |
