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Figure 2 Developmental expression of Xenopus NLK. (A) RT-PCR analysis shows that xNLK mRNA is maternal and expressed at all
stages of early development. Lane numbers correspond to developmental stages: 0, egg; 7, 8, 9, blastula; 10.5, 12, gastrula; 20, neurula;
24, tailbud tadpole; 34, swimming tadpole. (B) Whole-mount in situ hybridization shows the localization of the xNLK transcript. xNLK
transcript is distributed to the ectoderm in the early gastrula stages. Views of the vegetal pole ( left) and cross-section (right) are shown
and dorsal is right (a). At the neurula ( b) and tailbud tadpole (c) stage, xNLK expression is high in the central nervous system and head.
No signal is observed in a stage 32 embryo hybridized with a sense control probe (d). (C) Expression of xSox11 gene in the neural and
tailbud tadpole stages. Whole-mount in situ hybridization shows that the xSox11 transcript is high in the central nervous system and head.
Lateral views are shown. |
