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Fig. 4. Neural plate stage control expression patterns (A,D,G,J). (B,E,H,K) Overexpression of six1-WT (B) represses keratin, (E) increases the placodal domain of sox11 (inset, control side), (H) represses foxD3, but (K) has no significant effect on sox2 expression. The asterisks indicate the injected sides. (C,F,I,L) Six1-MO knock-down (C) expands the keratin domain closer to the border of the neural plate [np; compare bars on injected (*) versus control sides], (F) reduces the placodal domain of sox11, (I) increases the width of foxD3 domain, and (L) expands sox2 (bars indicate distance from midline). Quantitation of changes is presented in Table 1. (M) Injection of six1-myc results in protein expression detected by Myc antibody (green). (N) No protein (green) is detected when six1-MO is co-injected. (O) Same section as in N showing presence of lysamine-tagged six1-MO. (P) Injection of six1-rescue mRNA restores normal foxD3 domain on six1-MO injected side (*). |
