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Fig. 3. Increasing Wbp2nl levels alters ectodermal gene expression. (A) Increased Wbp2nl causes ectopic expression of epidermal keratin (K81) in the neural ectoderm (ne) at gastrula stages (left) but not at neural plate (np) stages (right). Pink nuclei identify the cells that are expressing excess Wbp2nl and the black arrow indicated the posterior extent of the clone. A-D, dorsal views with animal to top. (B) Increased Wbp2nl does not induce ectopic foxi1 in the ne. (C) Increased Wbp2nl causes ectopic expression of foxd3 in the neural plate (np). (D) Increased Wbp2nl causes ectopic expression of zic2 in the neural plate. (E-G) Increased Wbp2nl does not cause ectopic neural plate expression of pax3, zic1 or six1 (dorsal views with animal to top). In fact, zic1 neural plate expression is reduced on the injected side (F, right image, anterior view, dorsal to top). (H) Increased Wbp2nl does not alter foxd4l1 neural ectoderm expression. Vegetal-dorsal view. (I) Neural ectoderm expression of sox2 in the gastrula is reduced compared to lateral regions (black arrows) that do not contain Wbp2nl-expressing cells (pink nuclei). Dorsal view, animal to top. (J) Both the neural plate (red bar) and placode (red arrows) domains of irx1 are reduced by increased Wbp2nl compared to control side (black bar). Dorsal view, anterior to top. (K) Both the neural plate and placode (red arrows) expression of sox11 are reduced by increased Wbp2nl. Anterior view, dorsal to top. (L) The placode (red arrows) domain of six1 is reduced by increased Wbp2nl, compared to control uninjected side (black arrows). Side views, dorsal to top. (M) The placode (red arrows) domain of sox11 is reduced by increased Wbp2nl compared to control uninjected side (black arrows). Note reduced neural plate (np) staining as well. Side views, dorsal to top. Frequencies of the phenotypes and sample sizes (n) are given in each panel. |
