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Fig. 4. Whole mount in situ hybridization of gain and loss of function embryos. Embryos were injected with XDppa2/4, mDppa2, mDppa4 RNA or XDppaMO into both blastomeres at 2-cell stage. Whole mount in situ hybridization was performed at stage 11 for Xbra, BMP4, MyoD, Gsc, XSox2, Xema and XSox17a. Uninjected embryos or embryos injected with ctrlMO show the expected wild type expression pattern of marker genes at stage 11. Orientation of embryos is given. For the vegetal view the dorsal side is always located at top and for the lateral view the dorsal side is located at the left side. |
