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Fig. 5. PGCs deficient in Nanos1 activity express endoderm-specific genes. (A,C) RT-PCR analysis of PGCs or endoderm cells isolated from the endoderm core at stage 11 (circled in red, surrounded by endomesoderm in blue) (A) and stage 15 (C). P, primordial germ cells; E, endoderm; WE, whole embryo. Xpat is a germ plasm marker, Xsox17α an endoderm marker, Bix4 an endomesoderm marker and Xbra a mesoderm marker. ODC provides an internal control. The asterisk marks endoderm cell contamination. (B) Confocal analysis of WT, Nanos1-Ctrl-MO- and Nanos1-MO-injected embryos at stage 11. Xsox17α RNA (red) was detected by WISH and PGCs (outlined by dashed line) were identified by Xiwi immunostaining (green). Merged images are shown at the top, with separate channels beneath. Images were taken from the endoderm core. Scale bars: 50 μm. |
