| |
Fig. 7. In situ hybridization confirms differential expression of organ marker genes for stomach, lung, heart and kidney in LOF microarray. (A, B) Isolated whole gut NF42 showing expression of stomach marker agr2 almost entirely abolished in morphant tissue (n = 23). (C, D) Expression of stomach marker frp5 was reduced (n = 18). (E–J) Whole tadpoles at NF42 with ventral skin removed for in situ hybridization. (E, F) Lung marker surfactant C (SurfC) is decreased in morphant tissue, closed arrows (10/14 reduced). (G, H) Heart marker nkx2.5 is decreased in morphant tissue, open arrow (8/14 reduced). (I, J) Kidney marker kcnJ1 is increased in morphant tissue, arrowhead (8/11 increased). (K,L) Expression of liver marker hex is unaffected by rfx6 knockdown (n = 32). (M, N) Expression of gall bladder and duodenum marker hnf6 is reduced in duodenum but not gall bladder (17/18). (O, P) Gall bladder marker sox17α was not reduced (18/20 showed normal expression). |
