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Figure 4. Cdx1 activity is required for germ layer differentiation. To study the effect of Cdx1 manipulation on germ layer differentiation, embryos injected with Cdx1MO to induce an activity knockdown (B,E,H,K) or overexpressing Cdx1 (C,F,I,L) were analyzed during late blastula (st. 9.5) to study the earliest expression of the germ layer markers. The embryos were processed for whole mount in situ hybridization with probes for the early mesodermal markers eomesodermin (A–C) and Xbra (D–F) or the endodermal markers Sox17α (G–I) and mixer (J–L), and cleared for photography |
