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Fig. 4.
Gtpbp2 is required for embryonic ventrolateral mesoderm patterning. (A) Gtpbp2 is required for posterior tissue development. Diagram depicts the way 4-cell X. laevis embryos were injected. Colored figure is shown to illustrate how dorsal (D) and ventrally-fated blastomeres (V) are identified and injected. Dorsal is the lighter pigmented half. Grayscale figure is the lateral view of the embryo. Embryos were injected with 30 ng Gtpbp2 morpholino into the two ventral blastomeres at the 4-cell stage, placed in the ventral marginal zone (VMZ) and cultured to stage 35. Loss of Gtpbp2 leads to the complete loss of tail and posterior tissues. (B) Morpholino knockdown of Gtpbp2 results in a severe reduction of post (xpo), wnt8, and myod1 (myf3) expression. Vent1 expression domain is more restricted to ventral tissues compared to controls. Embryos were injected with 30 ng Gtpbp2 morpholino at 4 cell stage targeting the VMZ; the future posterior ventral tissues. (C) Gtpbp2 is not required for mesendodermal induction. Sox17a, mixer, and mix1 expressions are not affected. Embryos were injected bilaterally with 40 ng Gtpbp2 morpholino at the 2-cell stage. Three independent sets of at least 20 embryos were analyzed in B and C, resulting in similar effects on marker gene expression. |
