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Fig. 3.
bcar3 is ubiquitously expressed at the onset of gastrulation but becomes restricted to prospective notochord and ventral ectoderm by the end of gastrulation (A) Semi quantitative RT-PCR was used to analyze expression of bcar3, foxi1, t, sox17α and odc (as a loading control) in equivalent amounts of RNA from whole embryos (WE) or from ectoderm (EC), dorsal mesoderm (DM), ventral mesoderm (VM) or endoderm (EN) dissected from embryos at stage 10. Reverse transcriptase was omitted from one set of samples (-RT) as a control for genomic contamination. (B–M) Expression of bcar3, foxi1, t and sox17α was analyzed by in situ hybridization of probes to bisected embryos at stage 10, 11 and 13. Stage 10 and 11 embryos are oriented with dorsal to the right and stage 13 embryos are oriented with dorsal to the top. Arrows indicate ectoderm (blue), dorsal mesoderm (orange), ventral mesoderm (red) and endoderm (yellow).
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