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Figure 4. Effects of overexpression of Klf factors on embryonic development and germ layer formation. A–K: Injection of each 500 pg mRNA for Klf factors, as indicated in each panel, generated different effects on embryonic development. Lateral view is shown for uninjected control (Uninj.) and injected embryos. (Putative) dorsal is up and anterior is to the left. L: Ratios (percentages) of normal and defect embryos among the total numbers (n) of control (ctrl) embryos or embryos injected with Klf mRNAs. M: Analysis on the expression of t, sox17a and sox2 in control (ctrl) embryos and embryos injected with different Klf mRNA. Lateral view is shown for embryos with t expression, and animal pole is up. Both animal (a. view) and vegetal view (v. view) are shown for the embryos with sox17a expression. Dorsal view is shown for embryos with sox2 expression. N: Ratios (percentages) of the embryos with regular gene expression or misregulated gene expression among the total numbers (n) of control (ctrl) or injected embryos.Download figure to PowerPoint |
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Figure 7. Effects of knockdown of Klf factors on germ layer formation. A: Germ layer marker gene expression in embryos injected with different MOs. The doses of each injected MO were the same as those in Figure 6B–J. All embryos for t expression are shown as lateral view with animal pole being placed up. All embryos for sox17a expression are placed in vegetal view. All embryos for sox2 expression are shown as dorsal view with the anterior being placed to the left. B: Ratios of embryos with normal or altered gene expression among the total numbers (n) of injected embryos.Download figure to PowerPoint |
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Figure 9. Effect of double knockdown on gene expression. A: Effect of single knockdown and double knockdown of Klf2 and Klf7 on the expression of sox17a. B: Effect of single knockdown and double knockdown of Klf7 and Klf8 on the expression of gsc. C: Ratios of embryos with normal or disturbed transcription among the total numbers (n) of injected embryos. ctrlMO, K2MO, and K7MO were injected at 30 ng per embryo, and K8MO was injected at 50 ng per embryo. l, lateral view with animal pole to the top of each panel; v, vegetal view with dorsal side to the top of each panel.Download figure to PowerPoint |
