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Figure 1. In situ hybridization. A comparison of the whole-mount in situ hybridization procedure between Xenopus tropicalis and Xenopus laevis embryos. A: Whole-mount in situ hybridization with species-specific Sox17 alpha probes to laevis (left) and tropicalis (right) gastrula. A ventral view with dorsal up is shown. Probes penetrate more efficiently in tropicalis whole-mount hybridizations than they do in laevis embryos. Compare a midsagittal, Vibratome section of a laevis gastrula previously hybridized by whole-mount with a Sox17 alpha probe (B) with a laevis embryo for which the Sox17 alpha in situ hybridization was preformed on a paraffin section (C). Notice the lack of staining in the central deep endoderm of the sectioned laevis whole-mount. D: In contrast, a midsagittal, Vibratome section of a tropicalis gastrula, previously stained by tSox17 alpha , whole-mount shows complete penetration of the probe into the deep endoderm. E: A schematic of a midsagittal gastrula embryo. As in all the sections above, dorsal is to the left. Yellow indicates the deep and superficial endoderm, orange the mesoderm, and white the ectoderm. |
|
| Gene |
Clone |
Species |
Stages |
Anatomy |
|
sox17a
|
|
tropicalis
|
NF stage 10
to
NF stage 10.5
|
endoderm
,
superficial
,
deep
|
|
sox17a
|
|
tropicalis
|
NF stage 22
|
endoderm
,
midgut
,
hindgut
,
anterior
,
posterior
|
|
sox17a
|
|
tropicalis
|
NF stage 32
|
endoderm
,
gall bladder
,
glomus
,
aortic arch
,
vascular endothelium
|
Display additional annotations [+]
|
| |
Figure 5. Xenopus tropicalis Sox17 alpha / beta . In situ hybridization analysis of tropicalis embryos with Sox17 alpha (A-E) and Sox17 beta (F-J) antisense probes. Whole-mount (A,C,F,H) and sectioned (B,G) gastrula show that tSox17 alpha and tSox17 beta are identically expressed in throughout the deep and superficial endoderm (red arrows). D: At early tail bud stage (anterior to the left), tSox17 alpha is strongly expressed in the posterior endoderm but absent in the anterior endoderm, except behind the cement gland. I: By comparison at stage 22 (anterior to the left), tSox17 beta transcripts are almost absent from the entire endoderm, except behind the cement gland. E: At stage 32, tSox17 alpha expression persists in the extreme posterior endoderm, the gall bladder (g) precursors and in endothelial cells. J: In late tail bud stages, tSox17 beta is only expressed in a small patch of cells in the head. st, embryonic stage. |
