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Fig. S6. Characterization of Xenopus laevis Sox5 and Sox6. (A) Amino acid sequence alignment of Sox5 or Sox6 proteins from human (Homo sapiens) (Sox5, NP_008871; Sox6, NP_001139291), mouse (Mus musculus) (Sox5, NP_035574; Sox6, NP_035575), chicken (Gallus gallus) (Sox5, CAF25288; Sox6, XP_421000), frog (Xenopus laevis) (Sox5, AB682776; Sox6, AB682777) and zebrafish (Danio rerio) (Sox5, AAI63449; Sox6, NP_001116481). Identical and highly conserved amino acids are highlighted in black and gray boxes, respectively. Dashed lines indicate gaps. Two potential coiled-coil domains and the HMG box are underlined in blue and magenta, respectively. (B) The temporal expression patterns of xSox5 and xSox6 during embryonic development were analyzed by qRT-PCR. (C) Whole-mount in situ hybridization for xSox5 was performed on embryos at stages 15, 23, 29/30 and 35/36. NC, neural crest; PM, paraxial mesoderm; PSM, presomitic mesoderm; BA, branchial arch; VBWM, ventral body wall muscle. (D) Anterior and dorsal view of embryos shown in C. NC, neural crest; NF, neural fold; PM, paraxial mesoderm; OV, otic vesicle; BA, branchial arch; FB, forebrain; MB, midbrain; HB, hindbrain. |