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Fig. 4. Expression of cartilage maker gene, sox9, in tadpole and froglet blastemas. Limbs were amputated at the ankle level of tadpole hindlimb bud (A–C) or at the wrist level of froglet forelimb (D, E). (A) Expression in tadpole 3 days post amputation (dpa) blastema. Expression was observed in the proximal to amputation plane but not in the blastema. (B) Expression in tadpole 5 dpa blastema. Expression was observed in the proximal to amputation plane but not in the blastema. (C) Expression in tadpole 7 dpa blastema. Expression was observed distal and proximal to the amputation plane. (D) Expression in froglet 7 dpa blastema. Expression was observed in the blastema. (E) Expression in froglet 14 dpa blastema. Expression was observed in a broad region of the blastema. Lines indicate the estimated amputation planes. Distal is to the top in all figures. Scale bar = 400 μm. |
