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Fig. 4. The loss of RhoV differentially affects the induction of neural crest markers. Embryos were injected in one blastomere at the two-cell stage with 1 ng of T50N-RhoV mRNA and analyzed at the neurula stage for Sox9 (a), Slug (b) and Snail (c) expression. β-galactosidase mRNA was co-injected with T50N-RhoV mRNA to identify the injected side (red staining), oriented to the right in all panels. The loss of RhoV strongly reduced Sox9 (a) and Slug (b) expression on the injected side of the embryo while Snail (c) was not affected. |
