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Fig. 8. The phenotypes of Tbx6 morphants are rescued by Tbx1 plasmid injection. Alcian blue-stained cartilage from a stage 47 larva (A) or whole-mount in situ hybridization for Sox9 in a stage 27 embryo (B), injected with Tbx1/pCS2+ (10 pg) together with Tbx6 exint-MO. So, subocular; Et, ethmoid-trabecular; I, infrarostral; M, Meckel's cartilage; Q, quadrate; C, ceratohyal; Bh, basihyal; B, branchial. MO-injected sides (*) are shown by Magenta-GAL staining. Images of MO-injected sides are flipped horizontally to facilitate comparison. (C) RT-PCR analysis in stages 16, 22, and 28 embryos bilaterally injected with MOs alone (50 ng) or with Tbx6 or Tbx1/pCS2+ (20 pg). |
