| |
Fig. S4. snail2, snail1 and twist1 morphant phenotype in Xenopus tropicalis. (A-E) The C2 and C3 blastomeres of 32-cell X. tropicalis embryos were injected with snail2 (B,C), snail1 (D) or twist1 (E) MOs (10 ng/embryo). At stage 18, embryos were fixed and stained in situ for sox9 (A, control uninjected embryo); 60% (n=20) of the snail2 morphant embryos showed a decrease in sox9 expression, whereas only 12% (n=25) of the snail1 and 11% (n=35) of the twist1 morphant embryos displayed a similar decrease in sox9 expression. |
