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Figure 7. A: Histological analysis of a segmented spike. The section was stained with alcian blue, eosin, and hematoxylin. B,C: Analysis of molecular markers in the BMP4-implanted spike by section in situ hybridization. B: Gdf-5 expression. Although gdf-5 expression was still observed in the peripheral cartilage, remarkable expression was not observed in the joint-like region. C: sox-9 expression. Sox-9 transcriptions were detectable in the joint-like region. D,E: Immunoreactivity for type I and type II collagen. E: Decrease in the signals of type II collagen was observed in the joint-like region. Scale bar = 200 μm. |
