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Fig 6. Effects of sizzled over-expression.
The effects of sizzled over-expression were complex, as illustrated when the effects of injecting low (200 pgs/embryo) or high (600 pgs/embryo) amounts of sizzled-HA RNA into one blastomere of two cell embryos (control, uninjected side to the left in all images.) At stage 18, the effects on the expression of sox9 ((A)-control, (B)-low, (C)-high) and twist1 ((D)-control, (E)-low, (F)-high), snail2 ((G)-control, (H)-high), chd7 ((I)-high), and c-myc ((J)-control, (K,L)-high) were analyzed. In embryos injected with 200 pgs of Sizzled-HA RNA the sox9 expression domain was altered in 27 of 30 embryos (B); the twist1 expression domain was altered in 26 of 31 embryos (E); the snail2 expression domain was altered in 26 of 30 embryos (G); and the chd7 expression domain was altered in 19 of 24 embryos (data not shown). In embryos injected with 600 pgs of Sizzled-HA RNA, the sox9 expression domain was altered in 21 of 24 embryos (C); the twist1 expression domain was altered in 19 of 22 embryos (F); the snail2 expression domain was altered in 22 of 26 embryos (H); the chd7 expression domain was altered in 16 of 18 embryos (I); and the c-myc expression domain was altered in 24 of 28 embryos (K,L). Red-brown staining indicates lacZ staining due to co-injected LacZ RNA (used as a lineage marker). |
