Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
sox9xenopus prechordal plate [+] 

Too many results?Too few results?

Experiment details for sox9



Myosin-X is required for cranial neural crest cell migration in Xenopus laevis.

Good quality Poor quality
Gene Clone Species Stages Anatomy
sox9.S laevis NF stage 22 to NF stage 24 prechordal plate

Display additional annotations [+]
  Figure 3. MyoX knockdown inhibited cranial neural crest (CNC) migration and delays trigeminal nerve outgrowth. A: MyoX knockdown does not disrupt early induction of NC. Embryos were injected into one cell at the two-cell stage with 30-ng MO2 and 5 ng of fluorescently labeled control MO, cultured to stage 16/17, sorted using a fluorescent dissecting microscope to identify the injected side (on right in all cases shown, indicated by asterisks) and processed for whole-mount in situ hybridization with probes for mesoderm (myoD), neural plate (sox2), epidermis (keratin), and NC (snail2). No effect was observed. B: MyoX knockdown inhibits cranial NC cell migration. Embryos unilaterally injected at two-cell stage with Control MO (cMO) or MO2, along with nuclear-localized lacZ RNA for lineage tracing, followed by Red-Gal staining (Research Organics), then whole-mount in situ hybridization with Sox9 and Sox10 probes. St. 22; ventral migration of hyoid arch NC (black arrow head) and branchial arch NC (red arrow heads, dashed lines) was inhibited compared with the uninjected side (Sox9, 88%, n = 25; Sox10, 96%, n = 24.). The control MO had no effect. St. 24; migration into all three arches occurred but was still retarded on the MO2-injected side (Sox9, 83%, n = 30; Sox10, 79%, n = 29). Enlarged views of the relevant regions from the MO2-injected side are shown to the right. C: The trigeminal nerve was affected by loss of MyoX. Control MO or MO2 injected into one cell at the two-cell stage. By St. 32, the distal region of the trigeminal nerve (arrows on the uninjected sides - left) was absent on the MO2-injected side (88%, n = 25). The control MO (n = 26) had no effect. Percentages and standard deviation error bars indicated to right as bar graphs.
Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.14.0
Major funding for Xenbase is provided by grant P41 HD064556