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Figure 2. Xtsproutys and Xtspreds Are Expressed in Similar Patterns, but at Slightly Different Times(A) Cartoon depiction of domain structures for each protein. Spry, conserved Sprouty domain; EVH-1, VASP homology domain; KBD, c-Kit binding domain.(B) In situ hybridizations for each gene. At gastrula stages (stage 10.5), Xtsproutys showed more extensive staining around the blastopore than Xtspreds (arrow). Neurula stages showed increased staining of Xtspreds and decreased Xtsproutys. Tail bud stages showed similar staining in branchial arches (br), otic vesicle (ov), and tail bud (tb), but Xtspred staining was absent in mid-brain/hind-brain isthmus (mhb) and forebrain (fb).(C) Quantitative real-time RT-PCR analysis of each gene’s expression over time. Relative expression levels showed Xtsproutys peak before stage 12.5 and then diminish, while Xtspreds levels increased after stage 12.5. |
