|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
spry1
|
|
tropicalis
|
NF stage 28
|
forebrain
,
midbrain-hindbrain boundary
,
stomodeal-hypophyseal primordium
,
pharyngeal arch
,
tail bud
|
spry4
|
|
tropicalis
|
NF stage 28
|
otic vesicle
,
forebrain
,
midbrain
,
midbrain-hindbrain boundary
,
stomodeal-hypophyseal primordium
,
[+]
|
spry3
|
|
tropicalis
|
NF stage 28
|
spinal cord
,
trigeminal placode
,
supraorbital lateral line
,
trigeminal nerve
,
glossopharyngeal nerve
,
[+]
|
spry3
|
|
tropicalis
|
NF stage 15
|
neural plate
|
spry3
|
|
tropicalis
|
NF stage 23
|
spinal cord
,
posterior placodal area
|
spry3
|
|
tropicalis
|
NF stage 40
|
spinal cord
,
trigeminal ganglion
,
supraorbital lateral line
,
occipital lateral line
|
tlx3
|
|
tropicalis
|
NF stage 28
|
spinal cord
|
mnx1
|
|
tropicalis
|
NF stage 28
to
NF stage 32
|
spinal cord
|
|
|
Fig. 2. Temporal and spatial expression pattern of spry3. (A) Total RNA was isolated from Xenopus of the indicated stages and RT-PCR performed using oligonucleotides specific for the indicated genes. E, embryo; –RT, stage 26 without reverse transcriptase. odc is a loading control. (B) Comparison of the pattern of expression of spry1, spry2, spry3 and spry4 at stage 32. (C) Analysis of the spatial expression of spry3 during Xenopus development. Embryos at the indicated stages were fixed and subjected to whole-mount in situ hybridisation using a probe specific for spry3. (D) Sections (plane indicated in C) of embryos stained with the indicated probes, showing expression in differentiated motor and sensory neurons. |