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Fig. 7. In situ hybridization of dril1 morphants. Embryos were injected with either control morpholino (A–F) or dril1 morpholino (G–L). Whole NF13 embryos injected with 20 ng morpholino were stained for chordin (A and G) and Xbra (B and H). Sagittally bisected embryos injected with 30 ng morpholino were stained for frzb-1 (C and I), Fz7 (D and J), and prickle (E and K) at NF13, and for sprouty2 at NF12 (F and L). Symbols in C and I represent archenteron (a), blastocoel (b), dorsal lip of blastopore (white arrowhead), and ventral lip of blastopore (dark arrowhead). |