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Figure 2. Xsprouty2 is expressed dynamically during early development. Whole mount in situ hybridization of Xspry2 andXFGF8. (A) Vegetal view of gastrula stage embryos (stages 10–11.5). Xspry2 expression is induced in the dorsal marginal zone. Expression expands ventrally around the marginal zone during mid to late gastrula stages. (B) Xspry2expression in a stage 28 albino embryo and close-up of the head region.Xspry2 can be detected primarily in anterior neural structures, such as the mid-brain/hind-brain isthmus (mhb), otic vesicle (ov), forebrain (fb), and hatching gland (hg). Lateral expression is seen in the branchial arches (br) and the tail bud (tb). (C) Close-up of head region of a stage 28 embryo stained forXFGF8. XFGF8 has a similar expression profile toXspry2, although with more sharply defined boundaries. Black arrow indicates expression in the third branchial arch, which is absent from the Xspry2 expression profile. |
