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spry4xenopus forebrain 

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Experiment details for spry4

Characterisation of a new regulator of BDNF signalling, Sprouty3, involved in axonal morphogenesis in vivo.

Characterisation of a new regulator of BDNF signalling, Sprouty3, involved in axonal morphogenesis in vivo.

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Gene Clone Species Stages Anatomy
spry4 tropicalis NF stage 28 forebrain

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  Fig. 2. Temporal and spatial expression pattern of spry3. (A) Total RNA was isolated from Xenopus of the indicated stages and RT-PCR performed using oligonucleotides specific for the indicated genes. E, embryo; –RT, stage 26 without reverse transcriptase. odc is a loading control. (B) Comparison of the pattern of expression of spry1, spry2, spry3 and spry4 at stage 32. (C) Analysis of the spatial expression of spry3 during Xenopus development. Embryos at the indicated stages were fixed and subjected to whole-mount in situ hybridisation using a probe specific for spry3. (D) Sections (plane indicated in C) of embryos stained with the indicated probes, showing expression in differentiated motor and sensory neurons.