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Supplementry Fig. 2. (A) Vegetal view of a stage 11.5 embryo. The zygotic expression of Xenopus laevis Sulf1 is first detected in the mesoderm during mid-gastrula stage, but is excluded from the most dorsal mesoderm (arrowhead). (B) Dorsal view of a stage 14 embryo. Sulf1 is expressed in the paraxial mesoderm, but is excluded from the dorsal midline. (C) Lateral view of a stage 23 embryo. Sulf1 is expressed in the pronephros (arrowhead), somites and posterior mesoderm as well as anterior neural tissue. (D) Vegetal view of a stage 11.5 embryo hybridised to Xenopus laevis Sulf2. Zygotic Sulf2 expression is not detected. (E) Dorsal view of a stage 14 embryo. Zygotic Sulf2 expression is not detected. (F) Lateral view of a stage 23 embryo. Sulf2 expression is detected in a ventral region that could include haemangioblasts (arrowhead), in the mesendoderm underlying the cement gland (arrow), and in anterior neural tissue. (G) Vegetal view of a stage 11.5 embryo. The zygotic expression of Xenopus laevis6-O-sulfotransferase is only detected in endoderm as the punctuate staining indicated by the arrowhead. (H) Dorsal view of a stage 14 embryo. 6OST is expressed in the two bars in the presumptive hindbrain (arrowheads) and in the paraxial mesoderm. (I) Lateral view of a stage 23 embryo. 6OST is expressed in the endoderm (arrowhead) and in anterior neural tissues (arrow). |
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Supplementry Fig. 4. Sections taken through the eyes of stage 28 embryos subsequent to in situ hybridisation for Sulf2 and 6OST reveal the expression of Sulf2 and 6OST in the retina as indicated by arrows. |
