|
Gene |
Clone |
Species |
Stages |
Anatomy |
sulf2
|
|
tropicalis
|
NF stage 22
to
NF stage 23
|
midbrain
,
mandibular arch
,
eye primordium
,
anterior neural tube
|
sulf2
|
|
tropicalis
|
NF stage 26
|
midbrain
,
hindbrain
,
head region
,
ventral blood island
,
mandibular arch
,
[+]
|
sulf2
|
|
tropicalis
|
NF stage 28
|
midbrain
,
ventral blood island
,
mandibular arch
,
pineal gland
,
anterior neural tube
,
[+]
|
sulf2
|
|
tropicalis
|
NF stage 40
|
visceral pouch 1
,
visceral pouch
,
branchial arch 1
,
gill primordium
,
head
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
sox8
|
|
tropicalis
|
NF stage 22
to
NF stage 23
|
midbrain
,
roof plate
,
mandibular crest
,
hyoid crest
,
branchial crest
,
[+]
|
sox8
|
|
tropicalis
|
NF stage 26
|
midbrain
,
hindbrain
,
mandibular crest
,
hyoid crest
,
ventral blood island
,
[+]
|
fgf8
|
|
tropicalis
|
NF stage 22
to
NF stage 23
|
midbrain-hindbrain boundary
,
hyoid crest
,
hatching gland
,
tail bud
,
cranial neural crest
,
[+]
|
fgf8
|
|
tropicalis
|
NF stage 26
|
brain
,
midbrain-hindbrain boundary
,
hyoid crest
,
hatching gland
,
head region
,
[+]
|
fgf8
|
|
tropicalis
|
NF stage 28
to
NF stage 29 and 30
|
midbrain-hindbrain boundary
,
hatching gland
,
pharyngeal arch
,
hyoid arch
,
branchial arch
,
[+]
|
fgf8
|
|
tropicalis
|
NF stage 40
|
hatching gland
,
head region
,
visceral pouch 1
,
visceral pouch 3
,
visceral pouch 4
,
[+]
|
sulf1
|
|
tropicalis
|
NF stage 23
|
mandibular crest
,
presomitic mesoderm
,
posterior neural tube
,
paraxial mesoderm
,
pronephric mesenchyme
,
[+]
|
sulf1
|
|
tropicalis
|
NF stage 26
|
presomitic mesoderm
,
tail bud
,
paraxial mesoderm
,
pronephric mesenchyme
,
floor plate
,
[+]
|
sulf1
|
|
tropicalis
|
NF stage 28
to
NF stage 29 and 30
|
neural crest
,
presomitic mesoderm
,
somite
,
mandibular arch
,
hyoid arch
,
[+]
branchial arch
,
eye
,
floor plate
,
trigeminal ganglion
,
dorsal tail fin
,
pronephric kidney
,
neural tube
,
dorsal fin margin
,
ventral tail fin
,
intersomitic region
,
primary heart field
|
sulf1
|
|
tropicalis
|
NF stage 40
|
head region
,
branchial arch
,
branchial arch 1
,
branchial arch 2
,
branchial arch 3
,
[+]
|
fgf3
|
|
tropicalis
|
NF stage 22
to
NF stage 23
|
brain
,
hindbrain
,
midbrain-hindbrain boundary
,
mandibular crest
,
hyoid crest
,
[+]
|
fgf3
|
|
tropicalis
|
NF stage 26
|
brain
,
midbrain-hindbrain boundary
,
mandibular crest
,
tail bud
,
eye
,
[+]
|
fgf3
|
|
tropicalis
|
NF stage 28
|
brain
,
forebrain
,
midbrain-hindbrain boundary
,
mandibular crest
,
hyoid crest
,
[+]
|
fgf3
|
|
tropicalis
|
NF stage 40
|
head region
,
visceral pouch 3
,
visceral pouch 4
,
visceral pouch
|
|
|
Supplementary Figure 4: XtSulf-1 and XtSulf-2 are expressed in the migrating CNC and are flanked by FGF3 and FGF8 expression. Zygotic transcripts for XtSulf-1, XtSulf-2 , FGF3 and FGF8 are detected by whole mount in-situ hybridisation using DIG-AP labelled RNA in X. tropicalis embryos at NF stage 25 30 35 and 40, lateral views of embryos, anterior to the left. XtSulf-1 is expressed in the mandibular arch and somites throughout all tailbud stages, in the hyoid and branchial arch and pronephros from NF stage 25 till 40 in the primary heart field from NF stage 30 till stage 40. XtSulf-2 is expressed in the anterior mesendoderm, just ventral to the mandibular arch at NF stage 20 to 30, expression is then restricted to the gill at NF stage 40.
Frontal vibratome sections show XtSulf-1 expression in the pharyngeal mesoderm of the anterior and posterior branchial, hyoid and mandibular arches, XtSulf-2 expression in the pharyngeal endoderm of the mandibular arch and anterior ectodermal cleft of the hyoid arch, FGF3 and FGF8 expression in the anterior endodermal pharyngeal pouch of the posterior and anterior branchial, hyoid and mandibular arches.
Xenbase Curator notes: 1) bottom 2 panels, left side are labelled sox8 as are curated as such. 2) NF staging mis-identified in figure legend, and are curated as stage 22/23, 26, 28/29 and 40 respectively (left to right) - not 2,5 30, 35, and 40 as stated above) |