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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hs6st1
|
|
laevis
|
NF stage 28
to
NF stage 33 and 34
|
olfactory placode
,
brain
,
midbrain-hindbrain boundary
,
somite
,
eye
,
[+]
|
sulf1
|
|
laevis
|
NF stage 28
to
NF stage 33 and 34
|
hindbrain
,
somite
,
ciliary marginal zone
,
branchial arch
,
floor plate
,
[+]
|
sulf1
|
|
laevis
|
NF stage 28
to
NF stage 33 and 34
|
hindbrain
,
somite
,
ciliary marginal zone
,
branchial arch
,
floor plate
,
[+]
|
|
|
Fig. 1. Expression of Sulf1, Sulf2 and 6OST during tailbud stages in Xenopus laevis. (A) Lateral view of a stage 28 embryo hybridised to a probe for Sulf1A (BG408276). (B) Lateral view of a stage 34 embryo hybridised to a probe for Sulf1A. (C) Lateral view of a stage 28 embryo hybridised to a probe for Sulf2 (BG814048). (D) Lateral view of a stage 34 embryo hybridised to a probe for Sulf2. (E) Lateral view of a stage 28 embryo hybridised to a probe for 6OST (XL037105). (F) Lateral view of a stage 34 embryo hybridised to a probe for 6OST. Abbreviations: BA, branchial arches; CG, cement gland; CMZ, ciliary marginal zone; FP, floorplate; L, liver; LLP, lateral line placodes; MHJ, midbrain–hindbrain junction; NP, nasal placode; PN, pronephros; arrowheads denote some of the somites repeated along the embryonic axis. |
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Display additional annotations [+]
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Fig. 3. Complementary expression of Sulf2 and 6OST in the brain of Xenopus laevis embryos at stage 28. (A) Sulf2 is expressed in the ventral midbrain and hindbrain, the cement gland, the nasal placodes (red arrowheads), the retina, the midbrain–hindbrain junction, and the pineal gland (red asterisk). (B) 6OST is expressed in the dorsal part of the midbrain and hindbrain, but a reduced level of 6OST expression is found at the midbrain–hindbrain junction (MHJ). 6OST expression is also detected in the olfactory placodes, and in the three lateral line placodes posterior to the eye (red arrowheads). (C) A parasagital-section through the brain shows the ventral expression of Sulf2 in the midbrain and hindbrain. (D) A parasagital-section through the brain shows the tight restriction of 6OST expression to the dorsal part of the midbrain and hindbrain. (E) Double in situ hybridisation to En2 (blue) and Sulf2 (purple). Expression in the midbrain–hindbrain junction is indicated with an arrowhead. (F) Double in situ hybridisation to En2 (blue) and 6OST (purple). The midbrain–hindbrain junction is indicated with an arrowhead, and the gap in 6OST expression is indicated with an asterisk. (G) A section through the head of an embryo showing the expression of Sulf2 (purple) and En2 (blue) in the midbrain–hindbrain junction as indicated with an arrowhead. (H) A section through the head of an embryo showing the expression of En2 (purple) and 6OST (blue). The midbrain–hindbrain junction is indicated with an arrowhead, and the gap in 6OST expression is indicated with an asterisk. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hs6st1
|
|
laevis
|
NF stage 33 and 34
|
retina
,
lateral plate mesoderm
,
neural tube
|
sulf1
|
|
laevis
|
NF stage 33 and 34
|
otic vesicle
,
retina
,
floor plate
,
pronephric kidney
,
neural tube
|
sulf1
|
|
laevis
|
NF stage 33 and 34
|
otic vesicle
,
retina
,
floor plate
,
pronephric kidney
,
neural tube
|
|
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Fig. 2. Cross-sections of tailbud (stage 34) Xenopus laevis embryos hybridised to probes for Sulf1 (A and D), Sulf2 (B and E), and 6OST (C and F). Panels A–C are sections taken at the level of the otic vesicle. Panels D–F are sections taken at the level of the pronephros. Diagram shows level of sections taken. ov, otic vesicle; pn, pronephros. |