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sulf2xenopus cement gland 

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Experiment details for sulf2

Winterbottom EF and Pownall ME (2008) Assay

Complementary expression of HSPG 6-O-endosulfatases and 6-O-sulfotransferase in the hindbrain of Xenopus laevis.

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Gene Clone Species Stages Anatomy
sulf2.L laevis NF stage 28 to NF stage 33 and 34 cement gland

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  Fig. 1. Expression of Sulf1, Sulf2 and 6OST during tailbud stages in Xenopus laevis. (A) Lateral view of a stage 28 embryo hybridised to a probe for Sulf1A (BG408276). (B) Lateral view of a stage 34 embryo hybridised to a probe for Sulf1A. (C) Lateral view of a stage 28 embryo hybridised to a probe for Sulf2 (BG814048). (D) Lateral view of a stage 34 embryo hybridised to a probe for Sulf2. (E) Lateral view of a stage 28 embryo hybridised to a probe for 6OST (XL037105). (F) Lateral view of a stage 34 embryo hybridised to a probe for 6OST. Abbreviations: BA, branchial arches; CG, cement gland; CMZ, ciliary marginal zone; FP, floorplate; L, liver; LLP, lateral line placodes; MHJ, midbrain–hindbrain junction; NP, nasal placode; PN, pronephros; arrowheads denote some of the somites repeated along the embryonic axis.

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Gene Clone Species Stages Anatomy
sulf2.L laevis NF stage 28 cement gland

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  Fig. 3. Complementary expression of Sulf2 and 6OST in the brain of Xenopus laevis embryos at stage 28. (A) Sulf2 is expressed in the ventral midbrain and hindbrain, the cement gland, the nasal placodes (red arrowheads), the retina, the midbrain–hindbrain junction, and the pineal gland (red asterisk). (B) 6OST is expressed in the dorsal part of the midbrain and hindbrain, but a reduced level of 6OST expression is found at the midbrain–hindbrain junction (MHJ). 6OST expression is also detected in the olfactory placodes, and in the three lateral line placodes posterior to the eye (red arrowheads). (C) A parasagital-section through the brain shows the ventral expression of Sulf2 in the midbrain and hindbrain. (D) A parasagital-section through the brain shows the tight restriction of 6OST expression to the dorsal part of the midbrain and hindbrain. (E) Double in situ hybridisation to En2 (blue) and Sulf2 (purple). Expression in the midbrain–hindbrain junction is indicated with an arrowhead. (F) Double in situ hybridisation to En2 (blue) and 6OST (purple). The midbrain–hindbrain junction is indicated with an arrowhead, and the gap in 6OST expression is indicated with an asterisk. (G) A section through the head of an embryo showing the expression of Sulf2 (purple) and En2 (blue) in the midbrain–hindbrain junction as indicated with an arrowhead. (H) A section through the head of an embryo showing the expression of En2 (purple) and 6OST (blue). The midbrain–hindbrain junction is indicated with an arrowhead, and the gap in 6OST expression is indicated with an asterisk.