|
Gene |
Clone |
Species |
Stages |
Anatomy |
tbx3.L
|
|
laevis
|
NF stage 5 (16-cell)
|
animal hemisphere
|
tbx3.L
|
|
laevis
|
NF stage 9
|
animal hemisphere
|
tbx3.L
|
|
laevis
|
NF stage 10.5
|
mesoderm
,
involuted dorsal mesoderm
,
involuted ventral mesoderm
|
tbx3.L
|
|
laevis
|
NF stage 12.5
|
mesoderm
,
involuted ventral mesoderm
|
tbx3.L
|
|
laevis
|
NF stage 13
|
mesoderm
,
involuted ventral mesoderm
|
tbx3.L
|
|
laevis
|
NF stage 15
|
cement gland primordium
,
eye primordium
|
tbx3.L
|
|
laevis
|
NF stage 23
|
mesoderm
,
optic vesicle
,
heart primordium
,
ventral mesoderm
|
tbx3.L
|
|
laevis
|
NF stage 25
|
post-anal gut
,
trigeminal placode
,
optic vesicle
,
pronephric mesenchyme
,
heart primordium
,
[+]
|
tbx3.L
|
|
laevis
|
NF stage 32
|
olfactory placode
,
head mesoderm
,
post-anal gut
,
pineal gland
,
optic vesicle
,
[+]
|
tbx3.L
|
|
laevis
|
NF stage 33 and 34
|
olfactory placode
,
heart
,
post-anal gut
,
pineal gland
,
eye
,
[+]
|
tbx3.L
|
|
laevis
|
NF stage 37 and 38
|
heart
,
pineal gland
,
head
,
pronephric tubule
|
|
|
Figure 1. TBX3 Expression in Developing Mouse and Xenopus Embryos(A) Tbx3 (blue) in preimplantation embryos plotted with other mesendoderm class ESC genes (Oct4, red; Klf5, brown; Klf9, pink; T, violet). Data were obtained upon reanalysis of published data sets.(B) IF of E3.5 blastocyst for OCT4 (red) and TBX3 (green). Scale bars, 20 μm. Nuclei in DAPI (blue).(C–E) TBX3 expression in postimplantation mouse embryos. (C) Tbx3 mRNA in situ hybridization at E6.25 (prestreak) and E7.5 (midstreak). exVE, extraembryonic visceral endoderm. Arrows depict Tbx3-positive cells within the proximoposterior epiblast at E6.25 and mesoderm at E7.5. (D) Tbx3 mRNA in whole mounts of wild-type embryos at E7.75 and E8.25. (E) Z-section of an E6.5 TBX3-GFP reporter embryo. Nuclei in DAPI (blue). Scale bars, 100 μm.(F and G) tbx3 expression in Xenopus. (F) Temporal expression of tbx3 during early Xenopus embryogenesis. tbx3 transcripts are maternally supplied. Zygotic expression starts at stage 10 and increases during gastrulation. gapdh as loading control. Negative control represents a −RT reaction with gapdh. (G) Spatial expression of tbx3 at stages indicated during Xenopus development. tbx3 is detected in the invaginating mesoderm during gastrulation. White arrowheads, tbx3-positive ectodermal cells; red arrowheads, tbx3 expression in the developing cardiac region. m, mesoderm; cm, cardiac mesoderm. Scale bars, 0.5 mm.See also Figure S5. |