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Figure 6. Inhibition of Fgf signaling at the eye field stage triggers early changes in the expression and position of markers of retinal DV polarity. A–H: vax2 (A–D) and tbx3 (E–H) expression was assessed immediately following the inhibitor incubation period (A, B, E, F; approximately Stage 18) and at Stage 24 (C, D, G, H) in embryos treated for 5 hr at Stage 12 with control or SU5402 solutions. I, J: The intensity of the in situ hybridization label was scored from 1 (lightest) to 3 (darkest) and the percentage of the eyes receiving each score for vax2 (I) and tbx3 (J) is plotted for control and SU5402-treated embryos. The dorsal/ventral axis, cement gland (cg), and optic vesicle (ov) are labeled.Download figure to PowerPoint |
