|
Fig. 5: Nosip deficient Xenopus embryos show disturbed dorso-ventral
patterning in the retina.
A Schematic illustration of the measurement and results shown in B and C. The
retina is circled. A first dashed line defines the dorso-ventral eye midline. A second
dashed line from the nasal to the temporal side of the eyes was set perpendicular to
the dorso-ventral midline. A third, red line was used to highlight the border of marker
gene expression. defines the angle of the dorso-ventral shift in gene expression of
tbx3 and vax2. B and C tbx3 (B) and vax2 (C) expression by WMISH at stage 32 in
the eye. Close-up views are shown. In contrast to Control MO injection, Nosip MO
injection leads to a significant shift in dorso-ventral patterning of the retina.
Quantitative evaluations are shown. Abbreviations: n, number of independent
analysed embryos, n.s., not significant. Error bars visualize standard error of the
means (s. e. m.); *** p≤0.001. P-values are calculated by a nonparametric, one-tailed
Mann-Whitney rank sum test. |