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Figure 4. Expression pattern of Tbx5 in Xenopus tropicalis. The expression pattern of Tbx5 detected by in situ hybridization between late neurula and early tadpole stages is shown (uncleared except for g). a-j; Stages are as follows: stage 19 (a,b), stage 25 (c,d), stage 26 (e,f), stage 31/32 (g; cleared), stage 33 (h), and stage 40 (i,j). Except for the anterior view in b and the ventral view in j, embryos are oriented with anterior to the left. Anterior is to the top in j. ea, eye anlagen, hp, heart primordium, v, ventricle. |
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Figure 6. Cardiac expression of Tbx2, Tbx5, and Tbx20. The in situ hybridization patterns of Tbx2, Tbx5, and Tbx20 in the forming cardiac chambers were examined in transverse sections through the tadpole heart after looping. a: Tbx2 expression was seen in the myocardium of both the atrial (am) and ventricular (vm) regions of the looped heart at stage 36. b: Expression of Tbx5 was restricted primarily to the developing ventricular myocardium at stage 38. c: In contrast to Tbx5, high levels of Tbx20 expression were seen in the atrial region but not in the ventricle (stage 40). Original magnification, 00. |
