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Fig. 3. XatvMO disturbs the expression of mesodermal markers. (AL) 25 ng StdMO (A,C,E,G,I,K) or 25 ng XatvMO (B,D,F,H,J,L) were coinjected with 250 pg β-gal mRNA into (E-L) one or (A-D) two blastomeres of 4-cell stage embryos. The injected regions were visualized by red, indicating β-galactosidase activity. Injected embryos were grown to mid to late gastrulae for whole-mount in situ hybridization; signal is indicated by blue staining. Arrowheads indicate the XatvMO-injected region. (A,B) The expression of Xwnt8 (ventral view) and (C,D) Xbra (lateral view) was detected in the marginal zone, which was the future mesoderm of embryos injected with StdMO. However, the injection of XatvMO induced a dispersed expression into the animal region. (E-J) Dorsal view of late gastrulae ( Xbra) and mid gastrulae ( Xnot, Chordin). (E) Expression of Xbra, (G) Xnot and (I) Chordin was detected in the axial mesoderm of embryos injected with StdMO and was up-regulated in the XatvMO-injected region. (F) Xbra; (H) Xnot and (J) Chordin. (K,L) Vegetal view of mid gastrulae. The ectopic expression of Xatv mRNA was detected in the XatvMO-injected region (L), but not in the StdMOinjected region (K) . |
