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tbxtxenopus   

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Experiment details for tbxt

Peyrot SM et al. (2011) Assay

A revised model of Xenopus dorsal midline development: differential and separable requirements for Notch and Shh signaling.

Gene Clone Species Stages Anatomy
tbxt.L laevis NF stage 10 mesoderm , marginal zone
tbxt.L laevis NF stage 13 notochord , circumblastoporal collar , axial mesoderm

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  Fig.4. Ectopic Shh does not affect notochord formation and Notch signaling acts after specification to repress the notochord fate. Shh mRNA overexpression does not repress notochord specification at stage 10, assayed by chd (B) and Xbra (E), or notochord formation during gastrulation (Xbra, H). Activated Notch signaling does not affect notochord specification (C, F), but potently represses subsequent notochord formation (I). Pink is lacZ staining of co-injected lineage tracer.