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Fig 1
RARγ1 is required for the expression of mesoderm markers. (A-D) Embryos were microinjected unilaterally at the 2-cell or 4-cell stage with 3.3 ng RARγ1.S+3.3 ng RARγ1.L/S MOs (Fig. S3). Injected side is to the right of the dashed line, and is indicated by the magenta β-gal lineage tracer. WISH shows that RARγ1 MOs result in the loss of T, fgf8, wnt8 and gdf3. (E-H) Embryos were microinjected unilaterally at the 2-cell or 4-cell stage with 6.6 ng RARα2 MOs (Janesick et al., 2013). Injected side is to the right of the dashed line, and is indicated by the magenta β-gal lineage tracer. RARα2 MOs did not affect T, wnt8 or gdf3 expression, but did produce loss of N-tubulin as previously published (Janesick et al., 2013). All embryos, except in H, are shown in vegetal view at stage 10.5/11. H is shown in dorsal view, with anterior on the bottom at stage 14. Fractions represent the portion of embryos displaying the phenotype. NC, no change. |
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Supplemental Figure S1. RARγ1 is required for the expression of mesoderm markers.
Embryos were microinjected unilaterally at the 2-cell or 4-cell stage with 10ng RARγ.L/S MO (see Fig S3). Injected side is to the right of the dotted line, and is indicated by the turquoise β-gal lineage tracer. RARγ1 MO results in the loss of T (Brachyury) and Foxa4. |
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Supplemental Figure S3. Specificity of RARγ1 MO phenotype. Two different morpholinos were designed to target Xenopus laevis Rarγ1. (Top) Mapping of MOs to the .S and .L subgenomes (Session, 2016). MO#1 matches nearly perfectly to both .S and .L, whereas MO #2 matches only .S, and will not likely to bind .L. (Bottom) Embryos were microinjected unilat-erally at the 2-cell or 4-cell stage with 6.6 ng of either Rarγ1.L/S MO (#1) or Rarγ1.S (#2). Injected side is to the right of the dotted line, and is indicated by the magenta β-gal lineage tracer. RARγ1 MO #1 and MO#2 give the same knockdown phenotype on T and Znf703. Fractions represent the portion of embryos displaying the phenotype.
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