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Figure 5. The paraxial myogenic mesoderm is mispatterned in fgfr4 CRISPR embryos. (A–F, G–J) Dorsal-vegetal views showing expression of an array of mesodermal markers in stage 10 (A–F) and stage 12 (G–J) embryos. Expression of paraxial mesoderm markers myf5 and myoD is perturbed in fgfr4 CRISPR embryos. (K, L)
Xbra expression in embryos bisected through their dorsal midline at stages 10.5 and 12; red arrowheads point at xbra expression in the involuted mesoderm. Graphs show percentages of embryos with normal vs. abnormal expression of each marker; N = 32–40 embryos per Control or fgfr4 CRISPR; **p < 0.001, ***p < 0.0001. |
