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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
sox2
|
|
xenopus
|
NF stage 13
|
neuroectoderm
,
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
dorsal
|
sox2
|
|
xenopus
|
NF stage 21
|
neuroectoderm
,
trigeminal placode
,
neural plate
,
posterior neural tube
,
dorsal
,
[+]
|
sox2
|
|
xenopus
|
NF stage 15
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
krt12.4
|
|
laevis
|
NF stage 13
|
ectoderm
,
epidermis
,
non-neural ectoderm
|
krt12.4
|
|
laevis
|
NF stage 15
|
ectoderm
,
epidermis
,
non-neural ectoderm
|
krt12.4
|
|
laevis
|
NF stage 21
|
ectoderm
,
epidermis
,
non-neural ectoderm
|
|
|
Fig. 4. X. laevis FCN morphants do not express sox2. X. laevis embryos were injected in each blastomere at the 2 cell stage with 20 ng each of follistatin, chordin, and noggin MOS. Embryos were raised to the stage indicated, fixed in MEMFA, and stained for expression of sox2 or epidermal cytokeratin. (A) Some embryos expressed low levels of sox2, but this expression was not seen in a ring. Most FCN morphants do not express sox2. The fraction of embryos falling into each class of sox2 expression are shown in the lower right corner. (B) Cytokeratin is expressed strongly in FCN morphants at all stages assayed, but is excluded from the blastopore and adjacent tissue (22/24 embryos with strong circumferential expression). (C) Xbra expression is expanded in neurula stage FCN morphants. Embryos are shown in dorsal views with anterior to the top. |